Lab Protocols
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1. Radioisotope Orders

2. PAGE Gel Composition

3. Cesium chloride Plasmid Prep

4. DNA Sequencing

5. Synthetic DNA Deprotection

6. Synthetic RNA Deprotection

7. Standard Transcription Reaction

8. ASL Transcription Reaction

9. tRNA Transcription Reaction

10. Using Scintillation Counter

Company Protocols and Handbooks

Qiagen: RNA guidelines

Qiagen: Bacterial growth and storage

Qiagen: Bench Guide

Promega: In vitro transcription

Invitrogen: Amine modification

Stratagene: Quickchange

GE/Amersham: Phosphor Imager Screen

Protein Purification

New England Biolabs: IMPACT protein purification

Qiagen: His Tagged protein purification QIAexpressionist

 

 

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