Senior Research Technician, Bioanalytical Mass Spectrometry, UCSD Biomarker Discovery and Diagnostic CenterThe newly established UCSD Biomarker Discovery and Diagnostic Center seeks a research technician to implement analytical protein and peptide methods in support of basic and clinical investigators studying a wide range of human diseases. The facility currently houses two mass spectrometers (Orbitrap and LTQ with ETD) and a computer cluster for data analysis. This will be a senior position, responsible for executing experimental and analytical approaches for protein biomarker identification using HPLC and mass spectrometry (MS). The position requires working knowledge of HPLC and MS operation, maintenance, and troubleshooting. Managerial and strong communication skills are required. This is an outstanding opportunity for a scientist seeking analytical responsibility in the field of protein chemistry, proteomics, and biomarker discovery. UCSD is an outstanding research university with an excellent mass spectrometry and bioinformatics community. Duties and Responsibilities •Implement new and existing analytical methods for quantitative protein identification and post-translational protein modification analysis in biological samples, using solid phase, liquid, gel based and on-line extraction techniques, and quantitative HPLC, LC-MS, and LC-MS-MS methods as needed to support clinical and basic research projects. •Implementation of non-routine analytical work and assist other technicians doing routine analyses. •Help plan, implement and supervise (when appropriate) laboratory experiments to achieve scientific objectives outlined in grants and as directed by the PI. Active input into future approaches will be enthusiastically encouraged. •Analyze and prepare analytical results for publication. Write analytical method reports for inclusion in grant applications and publications. •Maintain appropriate quality controls, perform preventative maintenance, troubleshooting and minor repairs of analytical and scientific equipment, and supervise such work delegated to others. •Experience with MUDpit type approaches, including preparing of the fused silica columns is preferred. •Recommendations for new equipment purchases, and supervise installation of new analytical equipment.
The Senior Research Technician will carry out these duties independently under the general direction of the PI. She/he may provide assistance to other technicians and interact with various faculty investigators who collaborate with the laboratory. May also assist in identifying job responsibilities of junior technicians and graduate students, facilitate their recruitment and hiring, and supervise their work. Required Education and Qualifications •B.S. (Chemistry, Analytical Chemistry, Pharmaceutics) or equivalent degree and experience. •Minimum 4 years employment in quantitative analytical methods with demonstrable experience in protein identification and high-throughput productivity (LC-MS and MS-MS, HPLC). •Thorough knowledge of MS-MS operation, and experience with basic instrument maintenance and troubleshooting. •Ability to work independently and collaboratively, multitask different projects, take initiative, and identify and execute priorities. •Strong interpersonal and leadership skills, and outstanding verbal and written communication skills.
Send CV, cover letter, and three letters of reference to: Dr. Pieter Dorrestein Skaggs School of Pharmacy and Pharmaceutical Sciences and Departments of Pharmacology, Chemistry and Biochemistry Biomedical Science Building (BSB) Room 4090 9500 Gilman Drive, MC 0636 La Jolla, CA 92093-0636 E-mail: pdorrestein@ucsd.edu
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